N-2-羥乙基哌嗪-N-2-乙磺酸(HEPES),CAS:7365-45-9是一種氫離子緩沖劑,能較長時間控制恒定的pH范圍。
分子生物學級HEPES用于T4RNA連接酶進行RNA3'-末端標記,分離與分析核RNA之反應緩沖液組分、預雜交緩沖液緩和雜交緩沖液成分。
溶于水(2.25mol/L,0℃)。熔點210℃。紫外線吸光度A(5%/1cm,250nm,水中)<0.05。
Assay(Titration, Dry basis)
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≥99.0%
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Solubility 1M water
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Clear, Colorless solution
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pH 1%water solution 25℃
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4.8~5.6
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Water
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≤0.5%
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Infrared
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Complies
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Residue on ignition
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≤0.1%
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A280,0.1M Water
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≤0.035
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A260,0.1M Water
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≤0.045
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Appearance
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White crystalline powder
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