Directions
Dissolve 10.2 g of the medium in distilled water and adjust the volume to 1,000mL. Sterilize by autoclaving at 121°C for 15 minutes. Cool the sterilized medium to room temperature and add a proper amount of sterile 10% sodium bicarbonate. (pH will be 7.1-7.4 at 37°C, in an atmosphere of 5% CO2 in air by adding 18-30 mL of sodium bicarbonate solution.) Aseptically add 0.3 g Lglutamine and the desire amount or serum.

Summary
RPMI 1640 is a medium for suspension culture investigated by George E. Moore, former director of Roswell Park Memorial Institute. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization had been performed by filtration using a membrane filter. RPMI 1640 Medium “Nissui” 2 is an autoclavablepowdered medium specially manufactured to overcome the difficulties associated with filtersterilized media, such as contamination by Mycoplasma. This medium has growth promoting effect to primary cultures of mouse and human leukemia cells, various kinds of cell lines and KATO-III cells.
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date.
Quality of the product is not warranted after its shelf life.
When if medium or reagent touched eyes or mouth, immediately wash with plenty of water, and consult a physician. Any medium, reagent and materials must be sterilized by autoclaving or boiling water after use, and then disposed as industrial waste according to the law or regulations related to dispose.

Formula