Gel chromatography used to test Polymer standard

來源：seebio.cn作者：西寶生物人氣：-發表時間：2012-06-21 10:39:13【大中小】

Gel chromatography
gel-chromatography Gel chromatography separates bimolecular on the basis of size. This technique uses a molecular sieve in the form of polysaccharide gel that comprises spherical beads. As these beads are tiny and porous, so they allow only small molecules to enter the gel, while the larger molecules remain outside these beads. Therefore, when the column is eluted with an appropriate buffer, larger molecules pass out through the column. At a faster rate then smaller ones. This technique is also called as size exclusive chromatography.

Phases of chromatography
The components to be separated are distributed between two phases: a stationary phase and a mobile phase, which percolates through a stationary bed. The stationary phase basically comprises a column field with porous beads.
Column packing
Spherical porous beads of defined sizes are used to pack the chromatography column. The porous gel may comprise cross-linked dextrans (sephadex), cross-linked poly-acrylamide (bio- gel P) or cross-linked agarose (sepharose). When columns are created they are packed with porous beads with a specific pore size so that they are most accurate at separating molecules with similar to the pore size.

Method
A sample comprising a mixture of proteins is dissolved in the same solvent that is running through the column. The various molecules of this mixture get flushed through the column at different rates. The separation of these molecules depends on whether they can fit with in the pore size of packing material.

As a molecule flow through the column, it passes by a no. of porous beads. Small molecules enters the porous beads and hence, their rate of passage through the gel is retarded as compared to that of the larger molecule that can not fit into the pores and continue moving along the solvent. So, molecule with the larger size reaches the end of the column before the molecule with smaller size do. Small amount of solvent eluted through the gel are collected in different tubes and the protein present in each sample are analyzed.

Applications of the gel chromatography
Gel chromatography is a very efficient method for the separation, analyzing and characterization of the mixture of macro-molecules.
1. In nay chemical or bioprocessing industry, separation and purification of a product from complex mixture is very important. Gel chromatography can separate complex mixtures with great precision.
2. Chromatography is well suited to a variety of uses in the field of biotechnology, such as separating mixture of proteins.

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Product	Mw		Package
Polystyrene standard 162-20000K	LMW	162-5400	250mg
	MMW	6000-100k	250mg
	HMW	110k-1390k	250mg
	UHMW	2000k-20000k	250mg
Polymethyl methacrylate standard 820-610000K	LMW	700-85k	250mg
Polymethyl methacrylate standard 820-610000K	MMW and HMW	110-2100k	250mg
Polybutadiene standard		500-700k	250mg
Polyisobutene standard	LMW	226-1400	250mg
	MMW	3000-85k	250mg
	HMW	90k-436k	250mg
	UHMW	570k-2600k	250mg
Polycarbonate standard		4200-46100	1g
Polypropylene glycol standard		76-53000	1g
Polyvinyl acetate standard		17000-275000	1g
Polyvinyl chloride standard		21500-155000	200mg/1g
Polyvinyl butyral standard		66500-167000	1g
Polydimethysiloxane standard 162-10000K	LMW	162-35k	500mg
Polydimethysiloxane standard 162-10000K	MMW	40k-150k	500mg
Polypropylene standard 800-801000	LMW	800-34K	250mg
Polypropylene standard 800-801000	WMW	800-800K	1g
Polyethylene standard	LMW	800-34K	250mg
Polypropylene standard	WMW	800-800K	1g
Polyethylene standard 86-1165000	LMW	86-64K	1g
	UHMW	800K-115K	1g
	NMW	6K-115K	1g
	WMW low density	52K-150K	1g
	WMW High Density	102K	1g
Polyethylene terephthalate standard		27400-63500	1g
Polytetramethylene terephthalate standard		16150-54600	1g
Polytetrahydrofuran standard		1250-8950	1g
Cellulose acetate standard			250mg
Cellulose triacetate standard			250mg
Polycaprolactam（nylon 6）standard		17200-41000	1g
Polyamide（nylon 66）standard		32300-11萬	250mg
Polyacrylonitrile standard		85250-193100	500mg
P（VDF-HFP）standard		20萬-70萬	1g
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	HMW	300K-1700K	250mg
	WMW	1K-1200K	1g
Polymethacrylic acid standard	Set	1250-947K	250mg*10
Polymethacrylic acid standard	WMW	8K-690K	250mg
Polyacrylamide standard 3350-9000K	Non-ionic	3350-1100K	250mg
Polyacrylamide standard 3350-9000K	Non-ionic	3200-9000K	500mg
Polyethylene glycol standard 62-29450	LMW	62-1000	1g
Polyethylene glycol standard 62-29450	HMW	1K-30K	1g
Polyoxyethylene standard 24000-1750K	NMW	25K-1000K	250mg
Polyoxyethylene standard 24000-1750K	WMW	120K-2000K	1g
Polystyrene sulfonate standard	NMW	3K-5640K	250mg
Polystyrene sulfonate standard 1430-2850K	WMW	65K-700K	1g
Glucan(dextran) standard 180-7400K	LMW	180-36K	1g
	MWM	41K-145K	1g
	HMW	160K-440K	1g
	HMW	515K-1300K	1g
	UHMW	1900K-5900K	1g
	WMW	1K-6100K	1g
	Set	1270-676K	800mg*10
Hydroxyethyl starch standard 9600-2600K	LMW	96K-207K	250mg
Hydroxyethyl starch standard 9600-2600K	HMW	227K-575K	250mg
Hydroxyethylcellulose standard		4萬-6萬	250mg
Pullulan standard		5900-96萬	200mg
	Set	5900-788K	800mg*10
	Set	70K-825K	200mg
Polyvinyl Alcohol standard		5800-20萬	1g
Hydroxy propyl cellulose standard		12000-865000	250mg
		10K-50K	250mg
	WMW	30K-865K	250mg
	WMW	62700-94200	1g
Sodium carboxy methyl cellulose standard		109200-11600	1g
Polyvinylpyrrolidone standard		14400-350萬	1g
Poly2-vinylpyridine standard		3300-130萬	250mg
Poly Hydrochloride standard			1g
Chitosan standard		326700-141400	1g
Xanthan Gum standard			250mg
GUAR GUM standard			250mg