西寶生物提供常規生產高質量的乳膠微球、熒光微球、順磁性微球、鐵磁性微球和多種有色微球,這些微球表面可根據應用的需要添加不同的功能基團(如羧基和氨基等)。" />

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時間分辨熒光免疫分析用熒光微球

來源:seebio.cn作者:西寶生物人氣:-發表時間:2013-07-29 14:21:00【
時間分辨熒光免疫分析法是較先進的免疫分析方法之一,結合納米信號的放大作用,具有廣泛的應用前景,適用于腫瘤標志物、激素等高靈敏度免疫試劑盒的開發。西寶生物提供常規生產高質量的乳膠微球、熒光微球、順磁性微球、鐵磁性微球和多種有色微球,這些微球表面可根據應用的需要添加不同的功能基團(如羧基和氨基等)??捎糜冢?/div>
Fluorescence immunoassay 熒光免疫分析法;
Enzyme immunoassay (EIA) 酶免疫分析法;
Fluorescence microscopy 熒光顯微鏡法;
Confocal fluorescence microscopy 共聚焦熒光顯微鏡法;
Flow cytometry/ image cytometry 流式細胞術/成像細胞術;
Magnetic cell separation 磁性細胞分離;
Magnetic particles EIA 磁性微球酶免疫分析法;
Microfluidics 微流控技術;
Other research and industrial applications. 其他科研及工業應用等。
 
熒光微球(Fluorescent microsphere)作為一種特殊的功能微球,由于在單個微球中富集了能夠發射熒光的有機或無機物質,除具有無機物和有機物的性能外,同時在外界能量刺激下還能發射熒光。新近發展起來的熒光微球粒度均一、單分散性好、穩定性好、 發光效率高,微球表面弧度有利于抗原決定簇和抗體結合位點的暴露面處于較佳的反應狀態,因此在眾多領域都具有廣泛的應用,如生物化學、生物醫藥、臨床醫學、基因分析以及光學儀器等,其中熒光微球在醫藥、生物方面的應用尤為重要。
 
① 在標記和示蹤方面的應用
通過在聚合物微球表面或內部引入一種或多種熒光物質制備的熒光微球,早期被用作校準流式細胞儀和熒光顯微鏡的熒光準質微球,后逐漸被應用到細胞標記、生物分子標記及在活性條件下的示蹤,也可固定蛋白質分子等,并跟蹤其功能化過程。
 
② 在檢測方面的應用
將表面帶有能與被檢測物結合的配體的熒光微球與放射標記的被檢測物相混合,體系中能與微球結合的被檢測物,其放射能激發熒光物質產生熒光,而不能與微球結合的被檢測物,由于距離較遠,其放射能在水中淹滅,不能激發熒光,從而可以對不分離產物進行檢測。而且熒光微球能夠攜帶許多熒光分子,較弱的刺激就可以引發較強的信號,所以只需要少量的低能輻射就能產生熒光信號,避免了使用傳統的放射性微球造成的輻射危險,并在不損失檢測靈敏度的前提下降低了成本。
 
③ 在免疫分析、藥物篩選方面的應用
目前,熒光微球應用廣且有前途的就是生物醫學領域,國外已經產業化的熒光微球主要都是用于此領域。它主要包括高通量免疫分析、藥物篩選、固定化酶等。熒光微球可作為診斷試劑應用于免疫分析。熒光微球在生物樣品檢測中作為不同檢測對象的固定化載體,不同熒光微球對應不同的捕獲抗體,識別不同的抗原,從而實現多種待測抗原定性及定量檢測。
 
④ 在固定化酶、基因研究方面的應用
酶可以通過物理吸附或化學鍵合方式固定在熒光微球上,由于酶的立體形態被固定,使其不但具有較高的pH 穩定性、熱穩定性和貯存穩定性,而且易與反應物分離,可以重復使用,提高使用效率。因此,在微球載體上固定化酶或其它生物活性物質,更有利于其功能的發揮。
 
 
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1% w/v 2.0-2.4 μm
2ml
AEG0287A Fluorescent Particles, Light Yellow
淺黃色熒光微球
1% w/v 0.1-0.3 μm
2ml
AEG0287B 1% w/v 0.4-0.6 μm
2ml
AEG0287C 1% w/v 0.7-0.9 μm
2ml
AEG0287D 1% w/v 10.0-14.0 μm
2ml
AEG0287E 1% w/v 15.0-19.0 μm
2ml
AEG0306A Carboxyl Fluorescent Particles, CyGreen, Flow Cytometry Grade
羧基Cy綠色熒光微球
107/mL 3.0-3.4 μm
2ml
AEG0306B 107/mL 3.5-3.9 μm
2ml
AEG0306C 107/mL 5.0-5.9 μm
2ml
AEG0307A Carboxyl Fluorescent Particles, UltraBlue, Flow Cytometry Grade
羧基超藍色熒光微球,流式細胞級別
107/mL 3.5-3.9 μm
2ml
AEG0307B 107/mL 5.0-5.9 μm
2ml
AEG0309A Carboxyl Fluorescent Particles, Jade Green, High Intensity, Flow Cytometry Grade
羧基翠綠色熒光微球,高強度,流式細胞級別
107/mL 5.0-5.9 μm
2ml
AEG0310A Carboxyl Fluorescent Particles, Jade Green, Flow Cytometry Grade
羧基翠綠色熒光微球,流式細胞級別
107/mL 3.5-3.9 μm
2ml
AEG0310B 107/mL 5.0-5.9 μm
2ml
AEG0311A Carboxyl Fluorescent Particles, Pink, High Intensity
羧基粉色熒光微球,高強度
0.5% w/v 5.0-5.9 μm
2ml
AEG0312A Carboxyl Fluorescent Particles, Yellow
羧基黃色熒光微球
1% w/v 0.04-0.08 μm
2ml
AEG0313A Carboxyl Fluorescent Particles, Yellow,Low Intensity
羧基黃色熒光微球,低強度
1% w/v 0.7-0.9 μm
2ml
AEG0313B 0.5% w/v 5.0-5.9 μm
2ml
AEG0314A Carboxyl Fluorescent Particles, Pink,Low Intensity
羧基黃色熒光微球,低強度
0.5% w/v 5.0-5.9 μm
2ml
AEG0315A Carboxyl Fluorescent Particles, Nile Blue, Low Intensity
羧基黃色熒光微球,低強度
0.5% w/v 5.0-5.9 μm
2ml
AEG0316A Carboxyl Fluorescent Particles, Yellow, High Intensity
羧基黃色熒光微球,高強度
0.5% w/v 5.0-5.9 μm
2ml
AEG0321A Carboxyl Fluorescent Particles,NileRed
羧基尼羅紅色熒光微球
1% w/v 0.04-0.08 μm
2ml
AEG0322A Carboxyl Fluorescent Particles,NileRed, High Intensity
羧基尼羅紅色熒光微球,高強度
0.5% w/v 5.0-5.9 μm
2ml
AEG0323A Carboxyl Fluorescent Particles, Nile Blue, High Intensity
羧基尼羅藍色熒光微球,高強度
0.5% w/v 5.0-5.9 μm
2ml
AEG0324A Carboxyl Fluorescent Particles, Light Yellow
羧基淺黃色熒光微球
0.5% w/v 5.0-5.9 μm
2ml
AEG0325A Carboxyl Fluorescent Particles, Aqua Green, Flow Cytometry Grade
羧基水綠色熒光微球
107/mL 3.0-3.4 μm
2ml
AEG0325B 107/mL 3.5-3.9 μm
2ml
AEG0325C 107/mL 5.0-5.9 μm
2ml
AEG0327A Carboxyl Fluorescent Particles, UV/Light Yellow/Yellow
羧基熒光微球UV/Light Yellow/Yellow
1% w/v 0.04-0.08 μm
2ml
AEG0328A Fluorescent Carboxyl Magnetic Particles, Sky Blue
天藍色氨基熒光磁珠
1% w/v 1.0-1.4 μm
2ml
AEG0329A Fluorescent Particles, Sky Blue
天藍色熒光微球
0.25% w/v 0.04-0.09 μm
2ml
AEG0329B 0.25% w/v 0.1-0.3 μm
2ml
AEG0329C 1% w/v 0.4-0.6 μm
2ml
AEG0329D 1% w/v 0.7-0.9 μm
2ml
AEG0329E 0.2% w/v 10.0-14.0 μm
2ml
AEG0329F 0.2% w/v 15.0-19.0 μm
2ml
AEG0329G 1% w/v 1.7-2.2 μm
2ml
AEG0329H 1% w/v 2.5-4.5 μm
2ml
AEG0329I 0.25% w/v 7.0-7.9 μm
2ml
AEG0329J 0.2% w/v 2.5-4.5 μm
2ml
AEG0379A Carboxyl Fluorescent Particles, Yellow, Medium Intensity
中密度 羧基黃色熒光微球
0.5% w/v 5.0-5.9 μm
2ml
AEG0380A UltraRainbow Fluorescent Particles, Mid-Range Intensity
中密度超彩虹熒光微球
107/mL 3.5-3.9 μm
5ml
AEG0381A Fluorescent Particles, Pink,Medium Intensity
中密度粉色熒光微球
1% w/v 1.7-2.2 μm
2ml
AEG0382A Fluorescent Particles, Light Yellow,Medium Intensity
中密度淺黃色熒光微球
1% w/v 1.7-2.2 μm
2ml
AEG0383A Carboxyl Fluorescent Particles, Sky Blue, Medium Intensity
中密度天藍色羧基熒光微球
0.5% w/v 5.0-5.9 μm
2ml
AEG0384A Fluorescent Particles, Purple/Yellow,Medium Intensity
中密度紫色/黃色熒光微球
1% w/v 1.7-2.2 μm
2ml
AEG0385A Fluorescent Particles, Purple,Medium Intensity
中密度紫色熒光微球
1% w/v 1.7-2.2 μm
2ml
AEG0386A Fluorescent Particles, Purple/Yellow
紫色/黃色熒光微球
1% w/v 2.5-5.0 μm
2ml
AEG0387A Fluorescent Carboxyl Magnetic Particles, Purple
紫色氨基熒光磁珠
1% w/v 2.0-2.4 μm
2ml
AEG0388A Dimethylamino Fluorescent Particles, Purple
紫色二甲氨基熒光微球
1% w/v 2.5-3.5 μm
2ml
AEG0390A Fluorescent Particles, Purple
紫色熒光微球
1% w/v 0.04-0.06 μm
2ml
AEG0390B 1% w/v 0.1-0.3 μm
2ml
AEG0390C 1% w/v 0.4-0.6 μm
2ml
AEG0390D 1% w/v 0.7-0.9 μm
2ml
AEG0390E 1% w/v 10.0-14.0 μm
2ml
AEG0390F 1% w/v 15.0-19.0 μm
2ml
AEG0390G 1% w/v 25.0-35.0 μm
5ml
AEG0390H 1% w/v 2.5-4.5 μm
2ml
AEG0391A Fluorescent Particles, Aqua Green,Flow Cytometry Grade
水綠色熒光微球,流式細胞級別
107/mL 3.0-3.4 μm
2ml
AEG0491A 1%(10mg/ml) 0.02
1ml
AEG0493A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 0.1
1ml
AEG0495A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 0.2
1ml
AEG0497A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 0.5
1ml
AEG0499A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 1
1ml
AEG0501A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 2
1ml
AEG0503A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 3
1ml
AEG0505A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 4
1ml
AEG0507A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 5
1ml
AEG0509A Carboxyl Green fluorescent polystyrene BEADS
綠色羧基熒光聚苯乙烯微球
1%(10mg/ml) 6
1ml
AEG0511A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 0.1
1ml
AEG0512A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 0.1
1ml
AEG0512B 1%(10mg/ml) 0.2
1ml
AEG0513A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 0.5
1ml
AEG0514A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 0.5
1ml
AEG0515A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 1
1ml
AEG0516A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 1
1ml
AEG0517A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 2
1ml
AEG0518A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 2
1ml
AEG0519A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 3
1ml
AEG0520A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 3
1ml
AEG0521A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 4
1ml
AEG0522A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 4
1ml
AEG0523A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 5
1ml
AEG0524A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 5
1ml
AEG0525A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 6
1ml
AEG0526A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 6
1ml
AEG0527A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 8
1ml
AEG0528A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 8
1ml
AEG0529A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 10
1ml
AEG0530A Carboxyl Orangefluorescent polystyrene BEADS
橙色羧基熒光聚苯乙烯微球
1%(10mg/ml) 10
1ml
AEG0531A Orange fluorescent polystyrene BEADS
橙色熒光聚苯乙烯微球
1%(10mg/ml) 15
1ml
AEG0531B 1%(10mg/ml) 20
1ml
AEG0531C 1%(10mg/ml) 25
1ml
AEG0531D 1%(10mg/ml) 30
1ml
AEG0531E 1%(10mg/ml) 40
1ml
AEG0531F 1%(10mg/ml) 50
1ml
AEG0531G 1%(10mg/ml) 75
1ml
AEG0531H 1%(10mg/ml) 100
1ml
AEG0531I 1%(10mg/ml) 150
1ml
AEG0531J 1%(10mg/ml) 200
1ml
AEG0531K 1%(10mg/ml) 250
1ml
AEG0531L 1%(10mg/ml) 500
1ml
AEG0532A Carboxyl Red fluorescent polystyrene BEADS
紅色羧基熒光聚苯乙烯微球
1%(10mg/ml) 1
1ml
AEG0534A 1%(10mg/ml) 0.1
1ml
AEG0534B 1%(10mg/ml) 0.2
1ml
AEG0534C 1%(10mg/ml) 0.5
1ml
AEG0536A Carboxyl Near-Ir Fluorescent polystyrene BEADS
近紅外羧基熒光聚苯乙烯微球
1%(10mg/ml) 0.1
1ml
AEG0536B 1%(10mg/ml) 0.2
1ml
AEG0536C 1%(10mg/ml) 0.5
1ml
AEG0536D 1%(10mg/ml) 1
1ml
AEG0540A Near-Ir Fluorescent PLGA BEADS
近紅外熒光PLGA微球
  0.1
50 mg
AEG0540B   0.2
50 mg
AEG0540C   0.5
50 mg
AEG0540D   1
50 mg
AEG0540E   2
50 mg
AEG0540F   5
50 mg
AEG0540G   10
50 mg
AEG0540H   15
50 mg
AEG0540I   20
50 mg
AEG0540J   30
50 mg
AEG0540K   40
50 mg
AEG0540L   50
50 mg
AEG0540M   75
50 mg
AEG0540N   100
50 mg
AEG0540O   125
50 mg
AEG0540P   150
50 mg
AEG0540Q   200
50 mg
AEG0540R   250
50 mg
AEG0540S   300
50 mg
AEG0540T   500
50 mg
AEG0564A Fluorescent Pink Particle Kit, Odd # peaks
粉色熒光微球試劑盒
0.5% w/v 3.5-3.9 μm
6x1ml
AEG0565A Fluorescent Pink Particle Kit, Even # peaks
粉色熒光微球試劑盒
0.5% w/v 3.5-3.9 μm
6x1ml
AEG0575A Carboxyl Fluorescent Particle Kit, Pink, Even # peaks
羧基粉色熒光微球試劑盒,偶數
0.25% w/v 2.5-2.9 μm
6x1ml
AEG0575B 0.25% w/v 3.5-3.9 μm
6x1ml
AEG0576A Carboxyl Fluorescent Particle Kit, Pink, Odd # peaks
羧基粉色熒光微球試劑盒,奇數
0.25% w/v 2.5-2.9 μm
6x1mL
AEG0576B 0.25% w/v 3.5-3.9 μm
6x1mL

 

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