低取代度硫酸葡聚糖

來(lái)源：作者：人氣：-發(fā)表時(shí)間：2020-07-31 12:55:00【大中小】

低取代度硫酸葡聚糖

化學(xué)名稱(chēng)：Dextran, hydrogen sulfate sodium salt; Dextran sulphate; Sodium dextran sulfate; Dextran polysulfate
CAS 編號(hào)：9011-18-1

硫酸葡聚糖是由特定分子量的葡聚糖硫酸化反應(yīng)制得的。TdB生產(chǎn)的低取代度硫酸葡聚糖分子量從5 kDa到500 kDa。

硫酸葡聚糖是以鈉鹽形式呈現(xiàn)，并加入少量磷酸鹽作為穩(wěn)定劑。每個(gè)批次的產(chǎn)品都提供質(zhì)檢報(bào)告COA。嚴(yán)格仔細(xì)控制分子量程，含硫量，濕度等指標(biāo)。

結(jié)構(gòu)

葡聚糖是一種多糖，來(lái)源于腸系膜明串珠菌B512F，由α-D-(1-6)線(xiàn)性葡聚糖組成，側(cè)鏈含量較低（約5%），與葡萄糖的碳-3位相連。硫酸葡聚糖是特定分子量的葡聚糖硫酸化得到的衍生物。低取代度硫酸葡聚糖的硫酸組分在8-13%之間。

圖1。低取代度硫酸葡聚糖片段的結(jié)構(gòu)表征。

硫酸葡聚糖（DS）可按不同平均重均分子量，不同分子量分布，不同程度的取代度，不同陽(yáng)離子（例如鉀替代鈉）和不同硫酸化過(guò)程來(lái)生產(chǎn)。例如，僅憑硫酸葡聚糖70的名稱(chēng)無(wú)法完整識(shí)別該化合物，在評(píng)估這些化合物性能時(shí)應(yīng)考慮到這一點(diǎn)。

硫酸葡聚糖是由選定的葡聚糖片段和硫酸化試劑（例如氯磺酸）合成而來(lái)，反應(yīng)混合物經(jīng)過(guò)純化后，可以獲得白色粉末狀產(chǎn)品。

儲(chǔ)存和穩(wěn)定性

室溫下，硫酸葡聚糖在干燥密閉的容器內(nèi)可以保持穩(wěn)定超過(guò)6年。

應(yīng)用

硫酸葡聚糖涉及廣泛的應(yīng)用領(lǐng)域和性質(zhì)。舉例如下

細(xì)胞培養(yǎng)用的抗凝劑
脂蛋白的選擇性淀析
加速DNA雜交
從DNA-組蛋白復(fù)合物中釋放DNA
抑制tRNA與核糖體的結(jié)合
核糖核酸酶抑制劑
抗病毒性
用于化妝品的抗感染和透水保濕
分離微生物和大分子
疫苗佐劑
膜選擇性滲透研究

產(chǎn)品列表

產(chǎn)品編號(hào)	品名	分子量(kDa)	包裝
DB005-10g	Dextran sulfate 5 LS	5	10 g
DB005-100g			100 g
DB009-10g	Dextran sulfate 10 LS	10	10 g
DB009-100g			100 g
DB013-10g	Dextran sulfate 20 LS	20	10 g
DB013-100g			100 g
DB003-10g	Dextran sulfate 40 LS	40	10 g
DB003-100g			100 g
DB015-10g	Dextran sulfate 100 LS	100	10 g
DB015-100g			100 g
DB051-10g	Dextran sulfate 500 LS	500	10 g
DB051-100g			100 g

參考文獻(xiàn)

Möhwald, M. et al. Aspherical, Nanostructured Microparticles for Targeted Gene Delivery to Alveolar Macrophages. Adv Healthc Mater 6, ( 2017).

Dijk, M. et al. How Dextran Sulfate Affects C1-inhibitor Activity: A Model for Polysaccharide Potentiation. Structure 24, 2182–2189 (2016).

Shahraz, A. et al. Anti-inflammatory activity of low molecular weight polysialic acid on human macrophages. Scientific Reports 5, 16800 (2015).

Svensjö, E., Nogueira de Almeida, L., Vellasco, L., Juliano, L. & Scharfstein, J. Ecotin-Like ISP of L. major Promastigotes Fine-Tunes Macrophage Phagocytosis by Limiting the Pericellular Release of Bradykinin from Surface-Bound Kininogens: A Survival Strategy Based on the Silencing of Proinflammatory G-Protein Coupled Kinin B2 and B1 Receptors. Mediators of Inflammation https://www.hindawi.com/journals/mi/2014/143450/abs/ (2014) doi:10.1155/2014/143450.

Parraga, J. E., Zorzi, G. K., Diebold, Y., Seijo, B. & Sanchez, A. Nanoparticles based on naturally-occurring biopolymers as versatile delivery platforms for delicate bioactive molecules: An application for ocular gene silencing. International Journal of Pharmaceutics 477, 12–20 (2014).

Svensjö, E. et al. Maxadilan, the Lutzomyia longipalpis vasodilator, drives plasma leakage via PAC1–CXCR1/2-pathway. Microvascular Research 83, 185–193 (2012). Russo, L. M. et al. Renal Processing of Albumin in Diabetes and Hypertension in Rats. AJN 23, 61–70 (2003).

Landauer, K. et al. Influence of Carboxymethyl Dextran and Ferric Citrate on the Adhesion of CHO Cells on Microcarriers. Biotechnology Progress 19, 21–29 (2003). Hugerth, A. M. Micropolarity and Microviscosity of Amitriptyline and Dextran Sulfate/Carrageenan‐Amitriptyline Systems: The Nature of Polyelectrolyte–Drug Complexes. Journal of Pharmaceutical Sciences 90, 1665–1677 (2001).

Persson, B., Hugerth, A., Caram-Lelham, N. & Sundelöf, L.-O. Dextran Sulfate−Amphiphile Interaction; Effect of Polyelectrolyte Charge Density and Amphiphile Hydrophobicity. Langmuir 16, 313–317 (2000).

Hugerth, A. & Sundelöf, L.-O. Effect of Polyelectrolyte Counterion Specificity on Dextran Sulfate−Amphiphile Interaction in Water and Aqueous/Organic Solvent Mixtures. Langmuir 16, 4940–4945 (2000).

Burne, M. J. et al. Anomalous decrease in dextran sulfate clearance in the diabetic rat kidney. American Journal of Physiology-Renal Physiology 274, F700–F708 (1998).

Caram‐Lelham, N., Hed, F. & Sundelöf, L.-O. Adsorption of charged amphiphiles to oppositely charged polysaccharides—A study of the influence of polysaccharide structure and hydrophobicity of the amphiphile molecule. Biopolymers 41, 765–772 (1997).

Vyas, S. V., Burne, M. J., Pratt, L. M. & Comper, W. D. Glomerular Processing of Dextran Sulfate during Transcapillary Transport. Archives of Biochemistry and Biophysics 332, 205–212 (1996).

Wells, X. E. & Dawes, J. Role of the Liver and Kidney in the Desulphation of Heparin in vivo. Thromb Haemost 74, 667–672 (1995).

Vyas, S. V., Parker, J.-A. & Comper, W. D. Uptake of dextran sulphate by glomerular intracellular vesicles during kidney ultrafiltration. Kidney International 47, 945–950 (1995).

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