Glycosphingolipids (GSLs) are the most abundant and diverse class of glycolipids in animals (and are also present in fungi, plants, and invertebrates). Glycans present on GSLs have important roles in physiology and pathology. The ability to identify and measure GSLs is important for research in developmental neurobiology as well as lysosomal storage diseases such as Tay-Sachs and Gaucher’s disease. There is also growing interest in GSLs as possible targets for immunotherapy. Ceramide glycanase is an enzyme used to release glycans from GSLs to enable their characterisation. It cleaves glycans including GM1, GM2 and GM3 by cleaving the β-glycosyl linkage. Glycans can then be labelled using LudgerTag labelling technology.We have purified ceramide glycanase from Hirudo medicinalis and offer this in a kit along with buffer and GM1 glycolipid substrate.

● 鞘糖脂(GSLs)是動物中豐富和多樣化的糖脂類 (也存在于真菌、植物和無脊椎動物中)。鞘糖脂上出現的聚糖會在生理和病理上起到非常重要的作用。識別和測量鞘糖脂的能力在發育神經生物學以及溶酶體貯積癥中的研究是非常重要的-例如黑朦性癡呆和戈謝病等疾病。人們對作為免疫治療可能的靶標-鞘糖脂的興趣與日俱增。

● 神經酰胺聚糖酶用于從鞘糖脂上釋放聚糖使之能夠表征。它用β-糖基鏈切開包括GM1，GM2和GM3在內的聚糖。然后使用LudgerTag標記技術標記上聚糖。

● 我們有從醫用水蛭中提純的神經酰胺聚糖酶和提供與緩沖液一起的試劑盒以及GM1醣脂類基質。

Product Information:

Cat # LZ-CER-HM-KIT includes enzyme, buffer and GM1 glycolipid substrate

Ludger Products for Analysis of Complex Glycans of GSLs:

Cat# LT-KAB-A2 or LT-KAB-VP24 Ludger Tag? 2-AB Labeling Kit

Cat# LT-KAA-A2 Ludger 2-AA Labeling Kit

Cat# LC-S-A6 LudgerClean S cartridges

Cat# E-S001 Sialidase Alpha- (2-3,6,8,9)

Cat# CN-NA2-20U NA2 Glycan Internal Standard

Cat# CAB-GHP-30 2-AB Glucose Homopolymer Ladder

Cat# LS-N2-4.6 x 150 Ludger Sep? N2 High Resolution Amide HPLC Column

Cat# LS-N-BUFFX40 Ludger Sep? N Buffer 40X concentrate